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Flower Anther/ovary culture Shoot apical meristem culture Production of virus free germplasm Mass production of desirable genotypes Facilitation of exchange between ...
... Doing Tissue Culture Preparation of Medium Preparation of Medium Sterilization Laminar hood Hosta Crown and Buds 5 Distinct Phases Of Doing Tissue Culture Meristem ...
... conditions Usually a lab Sterile aseptic culture or MEDIA containing: Nutrients Sugars Vitamins Hormones Test tube Plant tissue: shoot tip, leaf section, meristem ...

Seed culture Embryo culture Ovary or ovule culture Anther and microspore culture In vitro pollination Organ culture Shoot apical meristem culture Somatic ...
Protoplast Culture: definition Isolated protoplasts have been described as "naked" cells because the cell wall has been removed by either a mechanical or an enzymatic ...
... DNA Surrounding histone protein: regulate gene Plant stem cells Shoot apical meristem ... skin Slide 13 Slide 14 Explant Plant stem cell and their propagation in culture ...
... Use high cytokinin and low auxin concentrations in leaf disc culture in cloning of Salpiglossis sinuata (Velvet Flower) Clonal Propagation of Orchids A-obtain a meristem ...
Meristematic tissues – localized regions of cell division Apical Meristems Primary or Transitional Meristem  Primary growth Protoderm  gives rise to epidermis ...
Calliclone (clones of callus), mericlone (clones of meristem) and protoclone ... variant Plantlets generated by the addition of herbicide to the cell culture system ...
Types of Culture In vitro of explant The non-dividing, differentiated, quiescent ... Cellular totipotency Plant tissue culture Embryo culture Seed culture Meristem culture ...
... line can give a continuous supply of young plants throughout the year Why do Plant Tissue Culture, II In plants prone to virus diseases, virus free explants (new meristem ...
... 14 cm) Flower Induction Requirements When bulbs are harvested, the apical meristem is ... bulbs are placed in each room Temperature Sequences Potted flowering tulip culture ...
... Contributes to cell enlargement (in leaves) Stimulates differentiation of cells (with auxins) High cytokinin and low auxin promotes shoot initiation in tissue culture ...
... Growth Processes What is Plant Tissue Culture? “… the aseptic culture of plant ... trace procambium tunica new leaf corpus pith cortex apical meristem axillary meristem ...
Cutting capable of regenerating roots (or shoots) from adventitious meristem. ... of nonclonal embryony.” “Micropropagation has many synonyms - tissue culture ...
... Day neutral (i.e. tomatoes) Floral stimulus is transmitted form leaves to meristem ... root initiation Weed control (2-4D) Inhibition of stem sprouting Tissue culture ...
... All plants that develop are transgenic Plant transformation using physical or biological methods requires a tissue culture stage In Planta Transformation Meristem ...
... Cells (Stem Cells) Primary Shoot Apical Meristem (SAM) Root Apical Meristem (RAM ... of NAA causes abscission Induction of Ethylene Synthesis Tissue Culture ...
... be down by taking a cutting from the stem (containing at least one meristem) and encouraging it to grow roots. More advanced methods include grafting and tissue culture.
... Transformation of Arabidopsis plants Dip floral buds in 1 ml of Agrobacterium culture for 5 to 15 min. Detergent added to allow bacteria to infiltrate the floral meristem.
Cradled between cotyledons is the apical meristem of the embryonic shoot. ... Test-tube culture makes it possible to regenerate genetically modified (transgenic ...
THERE IS A MERISTEM (LATERAL MERISTEM) BETWEEN THE XYLEM AND PHLOEM CALLED THE ... plant Stimulates morphogenesis (shoot initiation/bud formation) in tissue culture ...
The vascular cambium acts as a meristem for the production of secondary xylem and secondary phloem. The cork cambium acts as a meristem for a tough thick covering for ...
... natans This liverwort is amphibious and grows readily in laboratory culture. ... sporophyte is shaped like a tapered horn * The sporophyte has an intercalary meristem ...
Meristem was 1st to extract and purify active human haemoglobin in plant cells. ... protein manufacturing methods such as E. coli fermentation, mammalian cell culture ...

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